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anti β3 tubulin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti β3 tubulin
    Anti β3 Tubulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+%CE%B23+tubulin/pmc12992974-282-53-55
    Average 86 stars, based on 1 article reviews
    anti β3 tubulin - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: The following antibodies were used: anti-iNOS ( Ab178945 , abcam, USA), CD206 (E6TSJ, Cell Signaling Technology, USA), anti-IL-6 (bs-0782R, Bioss, Beijing, China), anti-TNF-α (YT4689, ImmunoWay, USA), anti-IL-1β (AF02479, AiFang biological, China), anti-GFAP (MA009369AOm, cusabio, China), anti-Iba-1 (17198, Cell Signaling Technology, USA), anti-HMGB1 (3935, Cell Signaling Technology, USA), anti-NeuN (24307, Cell Signaling Technology, USA), anti-β3-tubulin (45058, Cell Signaling Technology, USA), anti-GAP43 (8945, Cell Signaling Technology, USA), anti-CD31 (77699, Cell Signaling Technology, USA), Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (715-155-150, Jackson Immunoresearch, USA), CyTM3 AffiniPure Donkey Anti-Rabbit IgG (711-165-152, Jackson Immunoresearch, USA).

    Article Title: Lipid Metabolism-Driven CNS Repair via Targeted EV Delivery of PAF to Neurons.
    Article Snippet: The following antibodies were used: anti-GAPDH (GeneTex, GTX100118), anti-CD44 (R&D system, BBA10), anti-CD24 (Merck, SAB1402713), anti-CD63 (GeneTex, GTX132953), anti-HSP70 (GeneTex, GTX111088), anti-CD81 (GeneTex, GTX101766), anti-CNP (GeneTex, GTX103954) and anti- β3 tubulin (Cell Signalling Technology, CS5568).

    Article Title: Activation of the protective arm of renin-angiotensin system enhances mitochondrial turnover improving respiration and decreasing integrated stress response in a human Complex III deficiency model
    Article Snippet: The primary antibodies used were: anti-human OXPHOS cocktail (Abcam, ab11041); anti-PROHIBITIN (Abcam, ab28172); anti-CITRATE SYNTHASE (Abcam, ab96600); anti-MIC60 (Abcam, ab137057); anti-TOMM20 (Sigma-Aldrich, WH0009804M1); anti-phospho AMPK (T172) (Cell Signaling, 2535); anti-AMPK (Cell Signaling, 2532); anti-phospho FUNDC1 (S17) (Invitrogen, PA5-114576); anti-FUNDC1 (Aviva Systems Biology, ARP53280_P050); anti-phospho ULK1 (S638) (Cell Signaling, 14205); anti-ULK1 (Cell Signaling, 8054); anti-LAMP1 (Cell Signaling, 51774); anti-MasR (Santa Cruz Biotechnology, sc-390453); anti-GST-Tag (Cell Signaling, 2624); anti-LC3B (Cell Signaling, 3868); anti-CHOP (Cell Signaling, 2895); anti-LAMIN A/C (Santa Cruz Bio, sc-20681); anti-β3 TUBULIN (Cell Signaling, 5568); anti-VINCULIN (Sigma-Aldrich, V9131); anti-ACTIN (Sigma-Aldrich, MAB1501), anti-TOMM20 Alexa Fluor 488 (Abcam, ab205486); anti-GRP75 (NeuroMab, 75-127); anti-ATF4 (Cell Signaling, 11815); anti-PGC1α (Invitrogen, PA5-72948); anti-SDHA (Invitrogen, 459200); anti-phospho ACC (S79) (Abcam, ab68191); anti-ACC (Abcam, ab45174).

    Article Title: Lipid Metabolism-Driven CNS Repair via Targeted EV Delivery of PAF to Neurons.
    Article Snippet: The following antibodies were used: anti-GFAP (Merck Millipore, Mab360; 1:250 dilution), anti-Iba1 (Abcam, ab5076; 1:250 dilution), anti- β3 tubulin (Cell Signalling, 5568; 1:250 dilution), anti-SOX2 (Santa Cruz Biotechnology, sc-17 320; 1:250 dilution), anti-MAP2 (Merck Millipore, AB5622; 1:250 dilution), anti-NeuN (Merck Millipore, ABN78; 1:100 dilution) and anti-DCX (Santa Cruz Biotechnology, sc-8066; 1:250 dilution).

    Article Title: Lipid Metabolism‐Driven CNS Repair via Targeted EV Delivery of PAF to Neurons
    Article Snippet: The following antibodies were used: anti‐GFAP (Merck Millipore, Mab360; 1:250 dilution), anti‐Iba1 (Abcam, ab5076; 1:250 dilution), anti‐β3 tubulin (Cell Signalling, 5568; 1:250 dilution), anti‐SOX2 (Santa Cruz Biotechnology, sc‐17 320; 1:250 dilution), anti‐MAP2 (Merck Millipore, AB5622; 1:250 dilution), anti‐NeuN (Merck Millipore, ABN78; 1:100 dilution) and anti‐DCX (Santa Cruz Biotechnology, sc‐8066; 1:250 dilution).

    Incubation:

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 °C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), anti- TXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). .. Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 °C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), anti- TXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). ..

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury.
    Article Snippet: Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 ◦C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), antiTXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). .. Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 ◦C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), antiTXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). ..

    Article Title: Depletion of p75NTR in Schwann Cells Driven by Inflammation Mediates Cutaneous Pain in Psoriasis.
    Article Snippet: Proteins were separated by SDS-PAGE, transferred to PVDF membranes (Millipore, #ISEQ00010), and blocked with 5% BSA in PBST (0.05% Tween20) for 1 h at room temperature. .. Membranes were incubated overnight at 4◦C with primary antibodies (Anti-S100B (1:1000, sc-393919, Santa Cruz), anti-SOX10(1:1000, sc-365692, Santa Cruz), anti-MBP (1:1000, sc-271524, Santa Cruz), anti- β3-Tubulin (1:1000, #5566, Cell Signaling Technology), anti-p75NTR(1:1000, ab52987, ABcam), anti-NGF(1:1000, A14216, Abclonal), antiGM130 (1:1000, #70767, Cell Signaling Technology), anti-IL-17A (1:1000, 13082-1-AP, Proteintech), anti-p-TrkA (1;1000, #9141, Cell Signaling Technology), anti-TrkA (1:1000, #2505, Cell Signaling Technology), anti-c-Fos (1:1000, #2250, Cell Signaling Technology), anti-p-ERK1/2 (1:1000, #4370, Cell Signaling Technology), anti-ERK1/2 (1:1000, #9102, Cell Signaling Technology), anti-p-AKT (1:2000, #4060, Cell Signaling Technology), antiAKT (1:1000, #9272, Cell Signaling Technology), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-KEAP1(1:1000, 10503-2-AP, Proteintech), anti-Nrf2 (1:1000, #20733, Cell Signaling Technol- 15 of 18 e C om m ons L icense o a i a g b ( 4 T o a t G G S t w ( i e w r b w f a 4 C e E D m m ( C 4 A w d c u w i w e A Y Z 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , W iley O nline L ibrary on [06/04/2026]. .. Dermal tissue was rinsed in DMEM, minced, and digested in 2 mg/mL collagenase in DMEM (5 mL) at 37◦C for 2.5 h with Advanced Science, 2026 e g r d c c w a i 1 c s β w c o 4 P c c t S t r N e a p c 4 C t m 1 g C a s β ( A w p o ( A c t ( w t A s C i p A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , on [06/04/2026]



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    Santa Cruz Biotechnology β3 tubulin
    SH-SY5Y and U138 MG cell differentiation into neuron-like (NLCs) and astrocyte-like cells (ALCs). (A, B) Representative immunofluorescence images of <t>β3-tubulin</t> (in yellow) and Nestin (in green) for undifferentiated SH-SY5Y cells (Ctrl) and NLCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). Nuclei were counterstained with DAPI (blue). (C, D) Quantification of the fluorescence intensity for β3-tubulin and Nestin normalized to DAPI signals. (E) Relative mRNA expression levels of the proneural transcription factors Mash1 , Nurr1 , and NeuroD1 in undifferentiated and differentiated SH-SY5Y cells. (F) Representative S100B (in red) immunofluorescence images for undifferentiated U138 MG cells or ALCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). (G, H) Quantification of the U138 MG proliferating fractions in differentiated and control conditions measuring the EdU-incorporating cells. (I) Quantification of the S100B fluorescence intensity normalized to DAPI signals. Data are shown as mean ± SD from a minimum of three independent replicates (n ≥ 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *p< 0.03; **p< 0.02; ***p< 0.0002; ****p< 0.0001. Scale bar: 100μm. FI, Fluorescence intensity; Mash1, Mammalian achaete-scute homolog 1; NeuroD1, Neuronal differentiation 1; Nurr1, Nuclear receptor related 1 protein.
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    Image Search Results


    SH-SY5Y and U138 MG cell differentiation into neuron-like (NLCs) and astrocyte-like cells (ALCs). (A, B) Representative immunofluorescence images of β3-tubulin (in yellow) and Nestin (in green) for undifferentiated SH-SY5Y cells (Ctrl) and NLCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). Nuclei were counterstained with DAPI (blue). (C, D) Quantification of the fluorescence intensity for β3-tubulin and Nestin normalized to DAPI signals. (E) Relative mRNA expression levels of the proneural transcription factors Mash1 , Nurr1 , and NeuroD1 in undifferentiated and differentiated SH-SY5Y cells. (F) Representative S100B (in red) immunofluorescence images for undifferentiated U138 MG cells or ALCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). (G, H) Quantification of the U138 MG proliferating fractions in differentiated and control conditions measuring the EdU-incorporating cells. (I) Quantification of the S100B fluorescence intensity normalized to DAPI signals. Data are shown as mean ± SD from a minimum of three independent replicates (n ≥ 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *p< 0.03; **p< 0.02; ***p< 0.0002; ****p< 0.0001. Scale bar: 100μm. FI, Fluorescence intensity; Mash1, Mammalian achaete-scute homolog 1; NeuroD1, Neuronal differentiation 1; Nurr1, Nuclear receptor related 1 protein.

    Journal: Frontiers in Immunology

    Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

    doi: 10.3389/fimmu.2026.1792896

    Figure Lengend Snippet: SH-SY5Y and U138 MG cell differentiation into neuron-like (NLCs) and astrocyte-like cells (ALCs). (A, B) Representative immunofluorescence images of β3-tubulin (in yellow) and Nestin (in green) for undifferentiated SH-SY5Y cells (Ctrl) and NLCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). Nuclei were counterstained with DAPI (blue). (C, D) Quantification of the fluorescence intensity for β3-tubulin and Nestin normalized to DAPI signals. (E) Relative mRNA expression levels of the proneural transcription factors Mash1 , Nurr1 , and NeuroD1 in undifferentiated and differentiated SH-SY5Y cells. (F) Representative S100B (in red) immunofluorescence images for undifferentiated U138 MG cells or ALCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). (G, H) Quantification of the U138 MG proliferating fractions in differentiated and control conditions measuring the EdU-incorporating cells. (I) Quantification of the S100B fluorescence intensity normalized to DAPI signals. Data are shown as mean ± SD from a minimum of three independent replicates (n ≥ 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *p< 0.03; **p< 0.02; ***p< 0.0002; ****p< 0.0001. Scale bar: 100μm. FI, Fluorescence intensity; Mash1, Mammalian achaete-scute homolog 1; NeuroD1, Neuronal differentiation 1; Nurr1, Nuclear receptor related 1 protein.

    Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

    Techniques: Cell Differentiation, Immunofluorescence, Fluorescence, Expressing, Control

    Formation and morphological assessment of bi-hNSPHs. (A) Representative bright-field microscopy images showing the formation and growth of bi-hNSPHs within 14 days. Scale bar: 200 µm. (B) Growth curves based on the measurement of bi-hNSPHs areas (µm²) and diameters (µm) every other day. (C) H&E staining of bi-hNSPHs cryosection at 14 days to reveal their internal cellular disposition. Scale bar: 100 µm. (C) Representative immunofluorescence images of neuronal markers for SH-SY5Y – β3-tubulin in yellow, Synapsin in red, MAP2 in magenta and Nestin in green – and astrocytic markers for U138 MG – S100B in red. (D) MAP2, Microtubule associated protein 2.

    Journal: Frontiers in Immunology

    Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

    doi: 10.3389/fimmu.2026.1792896

    Figure Lengend Snippet: Formation and morphological assessment of bi-hNSPHs. (A) Representative bright-field microscopy images showing the formation and growth of bi-hNSPHs within 14 days. Scale bar: 200 µm. (B) Growth curves based on the measurement of bi-hNSPHs areas (µm²) and diameters (µm) every other day. (C) H&E staining of bi-hNSPHs cryosection at 14 days to reveal their internal cellular disposition. Scale bar: 100 µm. (C) Representative immunofluorescence images of neuronal markers for SH-SY5Y – β3-tubulin in yellow, Synapsin in red, MAP2 in magenta and Nestin in green – and astrocytic markers for U138 MG – S100B in red. (D) MAP2, Microtubule associated protein 2.

    Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

    Techniques: Microscopy, Staining, Immunofluorescence

    Internal composition of tri-hNSPHs. (A) Representative immunofluorescence images of tri-hNSPH cryosections at 4, 7, 9, 11, and 14 days of culture. Sections were stained for the astrocyte marker S100B (in red), the neuronal marker β3-tubulin (in yellow), and the microglia/macrophage marker Iba-1 (in cyan). Nuclei were counterstained with DAPI (blue). (B) Representative immuno-fluorescence images of neuronal – Synapsin in red, MAP2 in magenta and Nestin in green – and microglial – HLA-DR in red – markers on cryosectioned tri-hNSPHs. Scale bar: 50-100 µm. Iba-1, Ionized calcium-binding adapter molecule 1; MAP2, Microtubule associated protein 2.

    Journal: Frontiers in Immunology

    Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

    doi: 10.3389/fimmu.2026.1792896

    Figure Lengend Snippet: Internal composition of tri-hNSPHs. (A) Representative immunofluorescence images of tri-hNSPH cryosections at 4, 7, 9, 11, and 14 days of culture. Sections were stained for the astrocyte marker S100B (in red), the neuronal marker β3-tubulin (in yellow), and the microglia/macrophage marker Iba-1 (in cyan). Nuclei were counterstained with DAPI (blue). (B) Representative immuno-fluorescence images of neuronal – Synapsin in red, MAP2 in magenta and Nestin in green – and microglial – HLA-DR in red – markers on cryosectioned tri-hNSPHs. Scale bar: 50-100 µm. Iba-1, Ionized calcium-binding adapter molecule 1; MAP2, Microtubule associated protein 2.

    Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

    Techniques: Immunofluorescence, Staining, Marker, Fluorescence, Binding Assay